Protocol for evaluating ChatGPT in biomedical association generation and verification using a RAG-enabled, cross-model majority voting workflow

📅 2026-05-28
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🤖 AI Summary
This work addresses the challenge of hallucination in large language models when generating disease-related biomedical associations by proposing a verifiable and reproducible evaluation framework. The framework integrates retrieval-augmented generation (RAG), semantic matching with biomedical ontologies, majority voting across open-source large language models, and self-consistency analysis, thereby overcoming the limitations of traditional exact ontology matching and enabling multi-level semantic validation of generated content. Experimental results demonstrate that this approach significantly enhances the ability to assess the factual accuracy of associations produced by models such as ChatGPT, offering a novel paradigm for evaluating the reliability of biomedical knowledge generation.
📝 Abstract
We present a protocol to evaluate ChatGPT's ability to generate disease-centric biomedical associations. It outlines how we generate the associations, validate the biological entities using biomedical ontologies, and verify associations using literature. The protocol includes a self-consistency strategy to assess generative reliability across ChatGPT models. To address ontology exact-match limitations, we provide a use case performing semantic verification through a workflow enabled by Retrieval-Augmented Generation (RAG) powered by open-source large language models (LLMs). This enables LLMs to establish truth over content generated by other LLMs and expose hallucination.
Problem

Research questions and friction points this paper is trying to address.

biomedical association
ChatGPT evaluation
hallucination
entity validation
truth verification
Innovation

Methods, ideas, or system contributions that make the work stand out.

Retrieval-Augmented Generation
cross-model majority voting
biomedical association verification
hallucination detection
self-consistency evaluation
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